Biology Genetics Essay Example | Topics and Well Written Essays - 1750 words

Biology Genetics - Essay Example Ito ET. al. (1996) and Ryner et. al. (1996) shared that, sex differences in mating behavior and male sexual behavior among the Drosophila may be attributed to a single gene. Fru gene is created out of spliced transcripts from four distinct promoters. Lee et. al. (2000) added that, FruM is manifested in w2% of neurons in the Central Nervous System of male. These neurons are arranged into 21 distinct clusters in several regions of the ventral ganglia and brain, including some of the areas or regions implicated in male sexual behavior. Similarly, the complex behaviors of insects are related to the organization of their nervous system; the neural pathways from sensory to motor circuits must be properly explored to understand their behaviors; and focused also in the body electrophysiological nature (Lee, 2000). The male P1 transcripts according to Demir & Dickson (2005), encode male-specific proteins (FruM) which are both important and sufficient for male sexual behavior. The aforemention ed literatures and those which were cited under the discussions and implications are quite reliable which supported the study. This research postulated that there are several fruGAL4-expressing neurons which are interconnected in a circuit and are involved in male sexual behavior. This circuit can be named accordingly: Sensory, central, and motor components. Basically, the study is directed towards exploration o... It also intended to assess whether "fruGAL4 reproduces the endogenous pattern of fru P1 transcripts". To achieve these, the proponents conducted gene targeting for labeling and manipulation of neurons, whereby they particularly add the GAL4 exposed reading frame to the fru P1 transcripts. In other words, various GAL4-responsive UAS transgenes were utilized. In fact, they also have stained brains and anterior nerve cords from fruGAL4, UAS-nlacZ heterozygous males with anti- "-galactosidase (anti-"-gal) and anti- FruM antibodies. The membrane tethered GFP reporter (UAS-mCD8-GFP) was utilized to closely observe the actual projection patterns of fruGAL4 neurons among the males and to find out for the potential differences in these projections among the females. Aside from this, to find out whether fru GAL4 ORNs work in male courtship behavior, an FLP-in strategy analogous to that one being used for their anatomical characterization was employed.Single-pair courtship assays were also prep ared, in which males of these genotypes were joined to the wild-type virgin females. These assays were carried out in the dark in order to get rid of any visual cues that might affect or influence courtship, thereby increasing the male's reliance on chemosensory cues. Behavioral Assays, Immunohistochemistry and FLP-in-Silencing Constructs are all very powerful methods and fitted for the study. If we take a closer look on the assumptions and methods presented, the authors considered the classical as well as updated theories and principles for its variables. Thus, a very strong theoretical basis in the pursuit of the approaches or methods used has been established. Approaches utilized are congruent to the expected outcomes.